This product is used for lateral flow immunoassay to qualitatively
detect nasopharyngeal and oropharyngeal swabs of patients with
suspected infection.
Sample Requirements
[Sample requirements]
It is recommended to use the polyester sponge sample with PP
(polypropylene) rod for aseptic sample collection.
(1) Collection method of oropharyngeal specimens: The collection
head is slightly tilted, and both sides of pharyngeal tonsils are
exposed in the mouth. Wipe the pharyngeal tonsil at least three
times along the tongue root, and then wipe the posterior pharynx up
and down with cotton swabs at least three times.
(2) Methods of collecting nasopharyngeal specimens: The sampler
gently holds the recipient's head with one hand and the swab with
the other hand. Insert the swab into the nostril and slowly insert
it along the bottom of the inferior nasal meatus. Arc, don't use
too much force to avoid bleeding due to trauma. When the tip of the
swab reaches the back wall of the nasopharyngeal cavity, gently
rotate once (if it is a reflex cough), and then slowly take out the
swab.
(3) Sample treatment: the collected samples should be treated with
the sample buffer provided by the kit as soon as possible (if not
treated immediately, the samples should be stored in dry,
sterilized and sealed plastic pipes), and stored at 2 C ~ 8 C, with
long-term storage not exceeding 24 hours ~ -70°C, but repeated
freezing and thawing should be avoided.
Test Method
Before testing, all reagents were returned to room temperature. The
test should be carried out at room temperature.
I. sample extraction (see figure 1)
Add 400 L(~10 drops) of sample buffer vertically into the sample
extraction tube, then insert the sample into the tube and rotate it
against the inner wall for about 10 times, so that the sample can
be dissolved in the solution as much as possible.
2. Sampling along the inner wall of the extraction tube, leaving as
much liquid as possible in the tube, then taking it out and
discarding it.
3. Cover the dropper.
Ii test procedure (see figure 2)
1. Take out the test card from the sealed bag.
2. Add 2 drops (~80 L) of processed sample extract to the sample
well of the test card, and start the timer.
3. Place the test card at room temperature for 15 minutes and read
the results. The result is invalid after 20 minutes.
Interpretation of test results
Schematic diagram of card result judgment:
Invalid (1) Result: The quality control line (line C) has no
reaction line, and the test is invalid, so the test is repeated.
(2) Negative result: red ribbon, coloring of quality control line
(line C).
(3) Positive results: two red bands, the detection line (T line)
and the quality control line (C line) are colored.
